DNA-seq of an ELMSeq reporter cassette to study impact of C-terminal sequence variations on protein abundance
收藏NIAID Data Ecosystem2026-05-01 收录
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资源简介：
A ELMSeq reporter cassette was created to monitor Dam levels by methylation, and introduced in the genome. The regions of 6 nt upstream and 6 nt downstream the stop codon were randomized to study their effect on gene expression. The ELMSeq reporter cassette was composed of: promoter - dam - random N6 - stop codon TAA - random N6 - spacer - 4xGATC. The amplicon was spanning the C-terminal region. The cassette was introduced in Mycoplasma pneumoniae.
创建时间：
2023-10-13
