Long-term and seasonal evaluation on environmental microbiology and water quality of Shanmei reservoir in southeast China

As a crucial source of potable water, the quality of water in Shanmei reservoir hugely and directly impacts the safety and well-being of downstream residents. Microorganisms play a pivotal role in the reservoir's resource and energy cycle of the reservoir. However, ecological protection efforts for the Shanmei reservoir have encountered numerous challenges in recent years. This study conducted an extensive visual analysis of microbial communities in sediment from the Shanmei reservoir between 2022 and 2024 using amplicon sequencing technology targeting 16S rRNA gene. The results showed that the microbial diversity of sediment showed an obvious seasonal pattern. At the same time, microbial composition also changes with the long-term evolution of time, which may be closely related to the change in environmental conditions. In addition, we have also carried out long-term multi-dimensional monitoring of the water quality of the Shanmei reservoir, and the results show that the water quality has reached the national drinking water grade. In conclusion, this study not only unveiled the interseasonal dynamics and long-term evolutionary characteristics of sediment microbial communities but also elucidated the significant influence of environmental factors on their composition, structure and function. These findings offer a fresh perspective for understanding the freshwater ecosystem microbial dynamics and provide a scientific foundation for reservoir management and water quality protection.