Cell surface expression of BST-2 WT and ΔN20 variants.
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资源简介：
293T cells were co-transfected with 500 ng of VR1012, BST-2 WT or ΔN20 variant expression plasmids, along with 500 ng of pEGFP-N3 as a transfection marker. After 48 h, cells were harvested and stained with the anti-BST-2 mAb and Alexa 633 goat anti-mouse IgG, followed by flow cytometric analysis. Cells only transfected with pEGFP-N3 was used as a negative control. The samples were gated on EGFP+ cells, and the surface BST-2 levels are shown in histograms with mean fluorescent intensitiy values at the top right corner. This experiment was repeated for three times and the most representative data was shown.
创建时间：
2016-02-23
