Effects of high cholesterol diet on liver small RNA expression profile utilizing two different RNA-seq methods

Purpose: To reveal the small RNA profiles in the liver using traitional and PANDORA-seq methods Methods: 8 week old C57BL/6 male mice were fed a high cholesterol or low cholesterol diet for 4 weeks. Liver RNA was extracted by injecting Trizol into the aorta and collecting the flow through for further processing Results: PANDORA-seq revealed an abundant amount of tsRNA and rsRNA that were undetected by traditional-seq. Importantly, PANDORA-seq revealed that many differentially regulated sncRNAs also undetected with traditional-seq Overall design: Liver small RNA were sequenced from C57BL/6 male mice after 4 weeks of low cholesterol or high cholesterol feeding