Changes in cDC subpopulations after Flt3L treatment during mBSA arthritis.

A) Representative gating chart for splenic cDC subpopulations. Cells were gated on the total mononuclear cell population. The same gating strategy was used for cDC subpopulations in the lymph nodes. B) Representative FACS plots illustrating changes in splenic cDC subpopulations after Flt3L-treatment during mBSA arthritis. Frequencies give in quadrant gates indicate distribution of the three cDC subpopulations CD8+ (CD8+CD11b−), CD4+ (CD4+CD11b+) and CD4−CD8− (CD4−CD8−CD11b+) back gated on the total cDC (CD11cHIB220−) population. C) Relative increase (%) of splenic cDC subpopulations in Flt3-treated (day 10, n = 15 and day 28, n = 23) mice during mBSA arthritis as compared with sham-treated controls (day 10, n = 15 and day 28, n = 27). D) Representative FACS plots illustrating changes in cDC subpopulations in lymph nodes after Flt3L treatment during mBSA arthritis. Frequencies given in quadrant gates indicate distribution of the three cDC subpopulations as stated in B. E) Relative increase (%) in frequency of cDC subpopulations in draining lymph nodes of Flt3L-treated (day 10, n = 10 and day 28, n = 22) mice during mBSA arthritis as compared with sham-treated controls (day 10, n = 6 and day 28, n = 27). The relative increase was calculated as described in material and method section. Data are presented as mean ± SEM and statistical significance was assessed using unpaired t-test. *P<.05, **P<.01, ***P<.001.