aTotal number of reads that spanned V1. bNumber of reads used for analysis excluding reads with multiple deletions and inversions from sequencing error. cNumber of distinct nucleotide sequences among
High-throughput 3' DGE library preparation was conducted as per: dx.doi.org/10.17504/protocols.io.bumynu7w with 6 nucleotide barcodes on R2 for the 384 wells.Reads were demultiplexed using a cust
