Tests for involvement of free reactive oxygen species: formation of borneol, 5-ketocamphor and H2O2.*

*The experiments in Table 2 (except for entry 4) were performed on February 20, 2013 when the GC-MS was more sensitive to borneol detection than previous assays, due to installation of a new electron multiplier.Values are the average of 4 replicates ± S.E. 50 mM potassium phosphate buffer (pH 7.4) was used for all the assays and was sparged with argon (99%). Camphor was the substrate in all assays. Experimental details are included in Material S1.ND = Not Detected; N/A = Not Applicable.1The assay was performed using recombinant P450cam and m-CPBA as a shunt agent. 2 The assay was performed using recombinant P450cam, m-CPBA and catalase. 3 The assay was performed using recombinant P450cam, m-CPBA and glucose/glucose oxidase. 4 The assay was performed using recombinant P450cam, m-CPBA and superoxide dismutase. 5 The assay was performed using recombinant P450cam, m-CPBA and butylated hydroxytoluene. 6 The assay was performed using recombinant P450cam, m-CPBA and EDTA. 5 The assay was performed using recombinant P450cam, m-CPBA and butylated hydroxytoluene. 6 The assay was performed using recombinant P450cam, m-CPBA and EDTA. 7 The assay was performed using m-CPBA and ferrous sulphate.