RNA-seq of Arabidopsis root sieve element markers at 24h of iron deficiency

RNA-seq of pPEAR1::dBOX-YFP, pPEAR1-erGFP, and pC2-YFP to characterize the transcriptome associated with progressively more differentiated sieve elements. pPEAR1::dBOX-YFP is the proxy for early / phloem initials. pPEAR1-erGFP is the proxy for transit amplifying protophloem. pC2-YFP is the proxy for differentiation sieve elements. Each marker line was grown for 4 days in standard MS (Murashige & Skoog) media then transferred to iron deficient media (Murashige & Skoog containing ferrozine to chelate any iron present so that it's biounavailable). At 24h after transfer, roots were protoplasted and sorted using Fluorescence Activated Cell Sorting (FACS) to isolate GFP-positive or YFP-positive cells. Then, RNA-seq was performed in the sorted cell populations.