2018-2021年三极冰雪微生物后处理产品
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典型年三极冰雪微生物后处理产品收集了2010-2018年期间南北极以及青藏高原地区冰川、冰川雪和冰里采样细菌分析结果。通过整理归纳汇总得到三极区域土壤微生物后处理数据产品，数据格式为excel，方便用户查看。其中南北极和青藏高原地区冰川雪和冰里原核为刘勇勤老师实验组在2010-2018年间从NCBI数据库收集的细菌16S核糖体RNA基因序列。收集的序列通过使用DOTOUR软件计算序列之间相似度，相似度在97%以上的序列聚类为一个OTU，并定义OTU代表序列。OTU代表序列通过“Classifier”软件与RDP数据库进行比对，在可靠性大于>80%的情况下鉴定到属一级水平；青藏高原冰川采集时间为2010-2018年间，包含刘勇勤老师实验组分离的青藏高原7条冰川（珠峰东绒布冰川，天山一号冰川，古里雅冰川，老虎沟冰川，木孜塔格冰川，七一冰川和玉珠峰冰川），向述荣老师分离的马兰冰川和张新芳老师分离的若岗日冰川的细菌16S核糖体RNA基因序列。冰川样品采集后带回北京青藏高原院研究所生态实验室和兰州冰冻圈国家实验室，涂布平板后于不同温度下（4-25摄氏度）培养20天-90天并挑取单菌落纯化。分离的细菌提取DNA后以27F/1492R引物扩增16S核糖体RNA基因片段，并使用Sanger法测序。16S核糖体RNA基因序列通过“Classifier”软件与RDP数据库进行比对，在可靠性大于>80%的情况下鉴定到属一级水平。
The Typical Year Third Pole (Arctic, Antarctic and Tibetan Plateau) Snow and Ice Microbial Post-processing Dataset compiles bacterial analysis results from samples collected in glaciers, glacial snow and glacial ice across the Arctic, Antarctic and Tibetan Plateau regions during 2010–2018. A consolidated post-processing dataset of microbial communities in the Third Pole region was generated via sorting, induction and aggregation, with data stored in Excel format for convenient user access. The bacterial 16S ribosomal RNA (rRNA) gene sequences from glacial snow and ice in the Arctic, Antarctic and Tibetan Plateau were collected from the NCBI database by Professor Liu Yongqin’s research team between 2010 and 2018. Pairwise sequence similarities were calculated using the DOTUR software, with sequences sharing ≥97% similarity clustered into an Operational Taxonomic Unit (OTU), and a representative sequence was defined for each OTU. The OTU representative sequences were aligned against the RDP database using the "Classifier" software, and taxa were assigned to the genus level when the confidence score was ≥80%. Bacterial 16S rRNA gene sequences from Tibetan Plateau glaciers were isolated between 2010 and 2018, including sequences from 7 glaciers in the Tibetan Plateau studied by Professor Liu Yongqin’s team: East Rongbuk Glacier on Mount Everest, Tianshan Glacier No.1, Guliya Glacier, Laohugou Glacier, Muztagata Glacier, Glacier No.71 and Yuzhu Peak Glacier, as well as Malan Glacier studied by Professor Xiang Shurong and Ruogangri Glacier studied by Professor Zhang Xinfang. After collection, glacial samples were transported to the Ecological Laboratory of the Institute of Tibetan Plateau Research in Beijing and the State Key Laboratory of Cryospheric Sciences in Lanzhou. Samples were spread on agar plates, incubated at temperatures ranging from 4°C to 25°C for 20 to 90 days, and single colonies were picked for purification. Genomic DNA was extracted from the isolated bacteria, and the 16S rRNA gene fragment was amplified using the 27F/1492R primer pair, followed by Sanger sequencing. The obtained 16S rRNA gene sequences were aligned against the RDP database using the "Classifier" software, and taxa were assigned to the genus level when the confidence score was ≥80%.
