Chemical reprogramming of human blood cells into hCiPS cells [scRNA-seq]

Chemical reprogramming offers a fundamentally innovative approach for generating human pluripotent stem (hCiPS) cells using small molecules. Our recent studies showed that this approach was highly efficient in reprogramming human fibroblasts to hCiPS cells. In this study, we established a robust method that successfully generated hCiPS cells from both cord blood and adult peripheral blood cells. This method achieved efficient reprogramming with both fresh and cryopreserved blood cells. Overall design: Total of 5 scRNAseq samples were analyzed, which include PBMC EPC, S1D1, S1D3, S1D6, S1D8.