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Enzyme turnover numbers (k<sub>cat</sub>, s<sup>-1</sup>) and apparent catalytic activities (k<sub>app</sub>, s<sup>-1</sup>) of <i>R</i>. <i>toruloides</i> in batch cultivations on three different carbon sources–glucose (G), xylose (X) and acetate (A)—during exponential growth (exp) and nitrogen limitation (Nlim) phases.

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kcat values were retrieved from BRENDA using the GECKO Toolbox [17]. kapp values were obtained by dividing flux, mmol/gDCW/h, by protein abundance, mmol/gDCW. Flux refers to median from 2000 iterations of random sampling of the solution space [32]. Normalization of protein abundance refers to 80% recovery of sample injected. (XLSX)

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2023-04-26
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