CRISPR/Cas9 mediated deletion of the adenosine A2AR enhances CAR T cell efficacy [murine_CRISPR_A2AKO_RNA-seq]

Investigation of the transcriptional profile of stimulated murine CRISPR/Cas9 MOCK and A2ARKO a-HER2 CAR T cells in the presence/absence of NECA Overall design: Following CRISPR/Cas9 mediated editing of T cells with either an A2AR sgRNA or a non-targeting control (MOCK), cells were transduced to express the anti-Her2 CAR. After 7 days culture in IL-2 (100 U/ml) and IL-7 (200 pg/ml), MOCK or A2AKO CAR T cells were stimulated with plate bound anti-myc Tag (anti-CAR) antibody in the presence or absence of NECA (1 µM) for 8 hours. Cells were analyzed with 3' RNA-seq.