Effect of modulation of microRNA-455-3p on gene expression in SW1353 human chondrosarcoma cells

MicroRNA-455 is a key microRNA in cartilage, strongly induced by Sox9. In order to understand its function, we have overexpressed (miR-455-3p mimic) or inhibited (miR-455-3p inhibitor) miR-455 compared to appropriate controls and measured gene expression. SW1353 cells were plated in 6 well plates and grown for 24 hours. They were then transfected with miR-455 mimic, mimic control, inhibitor or inhibitor control at 50nM using Lipofectamine 3000 and incubated for 48 hours. RNA was purified using Trizol and treated with Dnase (TURBO, Ambion). We then performed RNASeq analysis on the cell-derived RNA from n=3 of each experimental condition