Supplement to "Sequenoscope: A Modular Tool for Nanopore Adaptive Sequencing Analytics and Beyond"

This dataset contains Supplementary <b>Tables S1–S30 </b>associated with the manuscript "Sequenoscope: A Modular Tool for Nanopore Adaptive Sequencing Analytics and Beyond". <b>Tables S1–S12</b> include sample manifest summaries, <b>Tables S13–S24 </b>include full sample manifests generated by the <b>analyze</b> module; and <b>Tables S25–S30</b> include summary tables generated by the<b> plot</b> module. All tables correspond to barcodes 1–6 from control and adaptive sampling conditions. These supplementary tables are referenced in the main text and provide expanded methodological context for the technical replicates.The sequencing summary file (<b>sequencing_summary.zip</b>) was produced during basecalling from POD5 raw signal data by MinKNOW and Guppy from ZymoBIOMICS Microbial Community DNA Standards, including the Even community (catalogue D6305) and Log community (catalogue D6306) microbial distributions. This file importantly contains the read termination reasons as part of the adaptive sampling run in <i>end_reason</i> column. This single file was generated for six barcoded libraries (barcodes 1–6), each sequenced under control and test adaptive sampling conditions (for more details see the Sequenoscope manuscript).This sequencing summary file was used by Sequenoscope to infer approximate per-read adaptive sampling outcomes (accepted, rejected, or no decision). The dataset supports reproducible evaluation of adaptive sampling performance in a defined mock metagenomic community.This <b>dataset </b>contains <b>18 </b>supplementary figures (<b>Figures S1–S18</b>) stored in <b>Supplementary Material - PDF_fixed.pdf</b> associated with the main Sequenoscope manuscript. The figures provide additional visualizations and technical validation results supporting the analyses presented in the study, including adaptive sampling decision dynamics, read length and quality distributions, coverage and abundance comparisons, and replicate-level summaries across control and adaptive sampling conditions. All figures were generated using the Sequenoscope <b>analyze</b> and <b>plot </b>modules and correspond to experiments performed on ZymoBIOMICS™ LOG and EVEN-distributed microbial community standards. These supplementary figures are referenced in the main text and provide expanded methodological context for the technical replicates.<br>