The effect of mouse cathelicidin (mCRAMP) on gene expression in CD4+ T cells cultured under Th17-driving conditions

We have shown that cathelicidin amplifies Th17 differentiation. To determine the mechanism through which this host defence peptide acts, we performed multiplex gene expression analysis (NanoString) to identify the effects of mouse cathelicidin (mCRAMP) on murine CD4+ T cells cultured under Th17-driving conditions. In the study presented here, whole single cell splenic suspensions were cultured under Th17-driving conditions, with or without mCRAMP (2.5 uM) for 24 hours. CD4+ T cells were isolated by FACS and the RNA extracted for NanoString analysis (mouse Immunology panel). We compared the gene expression profiles of untreated (UT) and mCRAMP-treated cells.