Cargo Sequestration

ESCRT-0 recruits ESCRT-I and thereby initiates the MVB pathway. Cargo Sorting ESCRT-I is a heterotetramer consisting of Vps23, Vps28, Vps37 (VPS37A, VPS37B, VPS37C or VPS37D), and Mvb12/Ubap1 (MVB12A, MVB12B or UBAP1). The UEV domain of Vps23 binds ubiquitinated membrane proteins. Vps28 interacts with the GLUE domain of Vps36 in ESCRT-II. Cargo Sorting ESCRT-II is a heterotetramer formed of Vps36, Vps22, and two Vps25 molecules. The GLUE domain of Vps36 binds PI3P, Vps28, and ubiquitinated membrane proteins. Vps25 interacts with Vps20 of ESCRT-III.

ESCRT-0招募ESCRT-I，从而启动了MVB途径。货物分类ESCRT-I是一种由Vps23、Vps28、Vps37（VPS37A、VPS37B、VPS37C或VPS37D）以及Mvb12/Ubap1（MVB12A、MVB12B或UBAP1）组成的异源四聚体。Vps23的UEV结构域与泛素化膜蛋白结合。Vps28与ESCRT-II中的Vps36的GLUE结构域相互作用。货物分类ESCRT-II是由Vps36、Vps22和两个Vps25分子组成的异源四聚体。Vps36的GLUE结构域与PI3P、Vps28和泛素化膜蛋白结合。Vps25与ESCRT-III的Vps20相互作用。