scRNA-seq analysis (well-based) of pharmacological HCN channel block with ZD7288 on rat primary cortical neuronal stem cells

We analysed transcriptomic changes of single cell transcriptomes from primary rat cortical neuronal stem cells (NSC) after pharmacologically blocking HCN channels with ZD7288 compared to untreated NSC. This well-based scRNA-seq aproach revealed a pronounced cell cycle arrest in G1 phase of ZD7288 treated (HCN blocked) NSC. scRNA-seq (well based) of two groups of neuronal stem cells, 4 replicates each group with a total of 500 cells: 200 treated 300 non-treated cells with addition of 5 wells containing bulk RNA (positive control) or 5 empty wells (negative control)