Expression dynamics, relationships, and transcriptional regulations of diverse transcripts in mouse spermatogenic cells

We were interested in how the complex mammalian transcriptomes were established. Using spermatogenesis as a model system, we address this question first by profiling the mRNAs, lncRNAs and circRNAs and examining their relative abundances in 4 types of SPCs (priSG-A, plpSC, pacSC, rST). Overall design: F1 pups of DBA/2 and C57BL6 mice were used for cell isolation and SSC culture. priSG-A, plpSC and pacSC were isolated from prepubertal mice undergoing the first wave of spermatogenesis while rST were from 60 dpp adult mice.