Comprehensive Expression Analysis of microRNAs and mRNAs in Osteoarthritic Synovial Tissue from a Mouse Model of Early Post-Traumatic OA [miRNA]

Our objective was to characterize disease-specific profiles of both microRNAs (miRNAs) and mRNA expression in synovial tissue from a mouse model of early post-traumatic OA to better understand the molecular processes involved in driving OA. OA was induced in 10-12 week old male C57BL6 mice by bilateral surgical destabilization of the medial meniscus (DMM). RNA was isolated from the anterior region of the synovium of DMM and sham-operated mice at 1 and 6 weeks post-surgery. Expression profiling was performed using Agilent miRNA and mRNA microarrays and validated by qPCR. OA severity was determined histologically in the contralateral joint.