Hvem expression in OT1 cells is required for their accumulation after LM-OVA infection.

5×104 purified naïve WT or Hvem−/− OT1 cells were adoptively transferred into CD45.1+ recipient mice. One day after the transfer, mice were orally infected with 1×109 LM-OVA and the amount of transferred OT1 cells was monitored at 7 or 70 days p.i. The percentage of CD45.2+ WT and Hvem−/− effector OT1 cells in the spleen, MLN and IEL of the recipient animals (A), as well as the absolute number of these cells in the spleen and MLN of infected mice (B), were measured 7 days p.i. Data shown correspond to 7 mice per group, pooled from two independent experiments. The percentage (C) and absolute number (D) of WT and Hvem−/− memory OT1 cells in the recipient mice were also calculated, 70 days after LM-OVA infection. Data shown are representative of two independent experiments. E-F- Co transfer experiments with equal numbers of naïve WT (CD45.1+CD45.2+) and Hvem−/− (CD45.2+) OT1 cells injected into CD45.1+ recipient mice. One day after co-transfer, mice were orally infected with LM-OVA and the percentage of WT and Hvem−/− cells gated on total OT1 cells, as well as the absolute number of both OT1 cell populations was calculated at 7 days (E) and 70 days (F) following infection. Data shown are representative of at least two independent experiments.