Hepatic tissue expression profiles of Acsm3 konckout and control mice.

Acyl-CoA synthetase medium-chain family member 3 (ACSM3) is one member of ACSM family and localized on the outer membrane of mitochondria, which catalyzes the activation of medium-chain (C4-C14) length FAs and xenobiotic carboxylic acids. We found that ACSM3 was lower expressed in the metabolic syndrome (MetS) patients and mice. ACSM3 mainly expressed in the liver. We speculated that ACSM3 deficiency may participated in the MetS progression and then constructed Acsm3 global knockout mice (C57BL/6J background) by CRISPR/Cas9 system. The knockout mice exhibited profound depletion of Acsm3 expression in liver extracts. Male mice are more sensitive to diet-induced MetS than female mice, and thus male mice were used in this study. The phenotype of Acsm3 knockout mice was analyzed with wild type littermates as controls. 12 weeks, livers from three male Acsm3 knockout mice and three male control mice were removed for RNA sequencing. To elucidate the molecular differences in hepatic expression profiles between Acsm3 knockout mice and control mice, at 12 weeks of age, we performed RNA sequencing analysis with three male Acsm3 knockout mice and three male control mice. Before that, all mice were housed under a 12 h light/dark cycle at a temperature of 24 °C and relative humidity of (56 ± 10) %, with free access to water and normal diet.