DNA methylation analysis of radiation-induced skin fibrosis of rats

This study aims to compared the genome-wide DNA methylation status in radiation-induced fibrotic skin and adjacent normal tissues of rats by methylated DNA immunoprecipitation sequencing (MeDIP-Seq). Male Sprague-Dawley (SD) rats (4 weeks old) were irradiated with a single 45-Gy dose of irradiation was administered to the treatment area at a rate of 750 cGy/min using a 6-MeV electron beam accelerator (Clinac 2100EX, Varian Medical Systems, Inc., CA). Skin tissues from nonirradated skin areas and irradietd areas were collected and subjected to DNA methylation analysis by MeDIP.