Methylation-sensitive SMiLE-seq sequencing data (illumina) of human transcription factors profiled in the Codebook/GRECO-BiT collaboration

We assayed 114 human transcription factors (TFs) for DNA binding using methylation-sensitive selective microfluidics-based ligand enrichment followed by sequencing (meSMiLE-seq). Across 23 experiments, we identified DNA binding sites for 48 TFs. Among these, 13 showed aversion towards methylated DNA, while 11 exhibited either a preference for methylated DNA or an alternative binding site including the modification. The DNA binding for the remaining TFs was unaffected by DNA methylation under the conditions of the assay.