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genome-wide CRISPR/Cas9 screen using survival to a NET-inducing stimulus

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NIAID Data Ecosystem2026-03-12 收录
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PLB-985 human myeloid cell line, differentiated (in RPMI 2.5% FCS, 0.5% dimethylformamide, P/S and glutamine) for 7 days. Mock-treated and PMA-stimulated for 16h to induce cell death via NET formation. Survivors were FACS-sorted for DNA extraction and sequencing

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2020-09-10
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