Gene expression in rat striatum following carbon monoxide poisoning and hypoxic hypoxia. Rattus norvegicus

We have proposed the existence of a threshold for brain damage, in terms of hydroxyl radical production in rat striatum, between poisoning of carbon monoxide (CO) at 1000 ppm and 3000 ppm, where blood CO-hemoglobin levels reach approximately 50% and over 70%, respectively. To search for factors involved in brain damage, we examined the effects of air, 1000 ppm CO, 3000 ppm CO and 5% O2 (hypoxic conditions comparable with those by 3000 ppm CO) on gene expression in rat striatum, using microarray analysis. Overall design: Gene expression was measured in rat striatum which had been obtained at 0, 2, 6, or 12 hours after the 40-minute exposure to air, 1000 ppm CO, 3000 ppm CO or 5% O2 (a rat for each time of each exposure). The control is 0 hour after air.