RNA sequencing of flow sorted Scgb1a1 lineage traced Control and Yap/Taz knockout lung epithelial cells

Proper lung function relies on precisely balanced numbers of specialized epithelial cell types that work together and are maintained in homeostasis. In this study we have described essential roles for the transcriptional regulators YAP and TAZ, which are key effectors of Hippo pathway signaling, in maintaining lung epithelial homeostasis. Phenotypes associated with Yap/Taz deletion include alveolar defects and a striking development of goblet cell metaplasia throughout the airways. Lineage specific deletion of Yap and Taz in Scgb1a1+ cells leads to increased Mucin production within the knockout cells in vivo. In order better characterize the in vivo transcriptional changes associated with Yap/Taz knockout in these cells, we have isolated Scgb1a1 lineage traced cells from control and yap/taz cNull mice and performed bulk RNA sequencing. Control and Yap/Taz cNull Scgb1a1CreER mice were administered ip tamoxifen for 5 consecutive days at approximately 8 weeks of age. Samples were analyzed 12 days from onset on tamoxifen administration. Lineage traced cells were isolated by FACS from collagenase-dissociated lung tissue and total RNA was isolated for sequencing.