Additional file 5 of The intrinsically disordered region of GCE protein adopts a more fixed structure by interacting with the LBD of the nuclear receptor FTZ-F1

Additional file 5: Figure S5. Subcellular localization of GCEC and FTZ-F1. The subcellular distribution of the YFP-GCEC-FLAG and YFP-LBD FTZ-F1 was analyzed 24 h after the transfection or co-transfection of the COS-7 cells. A) Representative images of the COS-7 cells expressing the YFP-GCEC-FLAG or CFP-LBD FTZ-F1 after single transfection. The YFP-GCEC-FLAG was observed in nuclei, while the CFP-LBD FTZ-F1 localized within the whole cell. B) Representative images of the COS-7 cells expressing the YFP-GCEC-FLAG and CFP-LBD FTZ-F1 after co-transfection. The YFP-GCEC-FLAG was still observed in the nuclei, while the CFP-LBD FTZ-F1 was shifted to a predominantly nuclear localization. C) Representative images of the YFP and CFP expression used as the control. Both proteins were observed within the whole cell.