Ageing of the hematopoietic stem cell (HSC) compartment is characterized by skewing towards the myeloid lineage and reduced stem cell function, the molecular basis of which is largely unknown. Using s
Background We previously identified a MUC5B gene promoter-variant that is a risk allele for sporadic and familial Idiopathic Pulmonary Fibrosis/Usual Interstitial Pneumonia (IPF/UIP). This allele was
Analysis of IL27RA- and IL27RA+ hematopoietic stem cell (lineage-, c-kit+ Sca-1+ CD150+, CD34-). The two population of hematopoietic stem cell (HSC) purified from the bone marrow of young (2 months) a
Human acute myeloid leukemia (AML) KG1a cells or mouse BM cells (mouse bone morrow cells were employed for each ChIP assay. The ChIP procedure was performed according to a previously described protoc