Generating MAS-friendly KASP markers suitable for CsCvy-1 selection in cucumber breeding

Cucumber is a widely grown vegetable crop plant and a host to many different plant pathogens. Cucumber vein yellowing virus (CVYV) causes economic losses on cucumber crops in Mediterranean countries as well as in some part of India and in African countries such as Sudan. CVYV is an RNA potyvirus transmitted mechanically as well as by whitefly (Bemisia tabaci) in a semipersistent manner. Control of this virus is heavily dependent on the management of the insect vector and breeding virus resistant lines. However, very few resistance sources against CVYV in cucumber exist, due to the lack of MAS-friendly molecular markers to these sources, which restricts the rapid generation of resistant lines. In this work, we generated MAS-friendly KASP markers suitable for CsCvy-1 selection in cucumber breeding using a segregating F2 mapping population and commercial plant lines, and genomics coupled with bulked segregant analysis method. Variant analysis was performed to generate SNP-based markers for mapping the population and genotyping the commercial lines. We fine-mapped the region by generating new markers down to 101 kb with eight genes. Some of the markers developed were polymorphic with all the commercial lines indicating the region is inherited in these lines and the marker could be incorporated into breeding programmes.