Dataset with segmentations of 75 stained cell images over P3HBV polymer films

We segmented 75 images of stained cells, divided in 5 folders according the thickness of the Poly-3-hydroxyvalerate films.

P3HBV polymer films of different thicknesses were prepared by casting solution technique.

For this, P3HBV was dissolved in chloroform to obtain homogeneous solutions with concentration of 1.0, 1.5, 2.0, 2.5 and 3.0%. The films were dried at room temperature for 48 hours in a dust-free environment, allowing the chloroform to evaporate completely and resulting in solid films. Upper surfaces of the films were used for cell cultivation.

Linear culture of mice fibroblasts NIH 3T3 was used to obtain the visual data of cell adhesion on P3HBV film samples. To assess the cytocompatibility of PHA samples, cells were seeded onto sterile polymer films samples at a density of 2 × 104𝑐𝑒𝑙𝑙𝑠/𝑐𝑚2 and cultured for 72 hours. After the incubation, the samples were washed with phosphate-buffered saline and cells were fixed with 4% paraformaldehyde solution.

Cell membranes were permeabilized with 0.2% Triton-X, and the cytoplasm was stained with fluorescein isothiocyanate, FITC (green) (Sigma-Aldrich, USA), for 1 hour in the dark at room temperature. The nuclei were visualized using 4’,6-diamidino-2-phenylindole, DAPI (blue) (Sigma-Aldrich, USA).

Cells were visualized using a Leica DMI8 fluorescent microscope with corresponding LAS X software.

Per each sample, we include: A raw image (.tif) with 2 subfolders containing the segmented masks of their cells and nuclei respectively.