Cloning efficiency and functional retention of T cell clones.

a Number of growing clones, percentage of wells seeded in brackets.b Number of clones producing>100 pg/ml more IFN-γ in response to autologous/antigen loaded autologous targets than to control targets, as a fraction of total clones tested; percentage in brackets. In some cases clones that did not proliferate long term were tested, so the denominator is different to the number of established clones.c CD4+ clones from LCL stimulation were pre-selected from a subset which were positive for antigen-stimulated proliferation, and thus may not be representative of the 62 proliferating clones.d Clones were tested for cytolytic activity (CD8+ clones from LCL and melanoma stimulation only), IFN-γ production (CD4+ and CD8+ clones from each stimulation), or proliferation (CD4+ LCL clones only). Data represent number of clones with any significant response to autologous antigen, as a fraction of total number of clones tested.ND, not done; – indicates no test additional to IFN-γ production was performed.Cloning efficiency and functional retention of T cell clones.