Small RNA profiling from cultured cells and tissues derived from Parkinson's disease patient [small RNA-seq]

High throughput sequencing of small RNAs (mature miRNA and piRNA/piRNA-like molecules) from control- and sporadic Parkinson´s disease patient derived fibroblasts (n=15), induced pluripotent stem cells (n=26) and differentiated midbrain neurons (n=10). In parallel, cingulate gyrus samples from eight control- and eight Parkinson´s disease patients obtained from the Netherlands Brain Bank were sequenced. We found significant differences between the groups in every cell type and in tissue. Two-condition experiment in three cell types and brain tissue: Control- and Parkinson´s disease patient derived fibroblasts, induced pluripotent stem cells and differentiated neurons were compared. Fibroblast cell cultures were established from skin biopsies from six control patients and nine sporadic Parkinson´s disease patients and reprogrammed with the OSKM cocktail. Small RNA libraries were prepared with the TrueSeq Small RNA kit (Illumina) and sequencing was performed on an Illumina HiSeq1000 instrument (1x50 bp). Differential expression analysis was performed comparing control- and Parkinson´s disease patient derived cells within each cell type (fibroblasts, induced pluripotent stem cells or neurons) with DESeq2. Please note that Raw data is only available via dbGAP due to privacy concerns