The neurodevelopmental genes alan shepard and Neuroglian contribute to female mate preference in African Drosophila melanogaster

Mate choice is a key trait that determines fitness for most sexually reproducing organisms, with females often being the choosy sex. Female preference often results in strong selection on male traits that can drive rapid divergence of traits and preferences between lineages, leading to reproductive isolation. Despite this fundamental property of female mate choice, very few loci have been identified that contribute to mate choice and reproductive isolation. We used a combination of population genetics, quantitative complementation tests, and behavioral assays to demonstrate that alan shepard and Neuroglian contribute to female mate choice, and could contribute to partial reproductive isolation between populations of Drosophila melanogaster. Our study is among the first to identify genes that contribute to female mate preference in this historically important system, where female preference is an active premating barrier to reproduction. The identification of loci that are primarily know..., , , # Data From: The neurodevelopmental genes alan shepard and Neuroglian contribute to female mate preference in African Drosophila melanogaster

[https://doi.org/10.5061/dryad.cvdncjtbg](https://doi.org/10.5061/dryad.cvdncjtbg)

## Description of the data and file structure

In this experiment, we wanted to find genes important for female mating behavior that contributes to female preference between populations of Drosophila melanogaster.

### Cline Analysis

We first started by conducting a population genetic analysis using clinal analysis from publicly available data. The files and scripts used in this analysis are contained in the two folders with contents described here

Folder: cline_snp

This folder contains the initial analysis of the clines including the following files

snp_freq.R  This file splits the raw genotype data into subfiles. This was a necessary step to make sure we could analyze the clines in parallel. We focused on separating snps that had fixed differences vs segrega...