FT-IR/ATR determination of secondary structure percentages of N and B isomers of HSA and its uremic complexes.

aNormal condition: HSA complexed with creatinine under normal serum condition.
bUremic condition: HSA complexed with creatinine under maximal uremic condition.
These complexes were obtained incubating uremic toxin with 500 µM of HSA for 1 hr at room temperature. Data represent average obtained from two independent replicates, standard error is indicated.