Analysis of the nuclear proteome fraction of rAAV2-plasmid transfected HEK-293 cells during AAV production

In this comparative proteomic analysis, we focused on proteins in the nuclear fraction. As the nucleus is the epicenter of DNA uptake, transcription, capsid assembly and packaging. Three HEK-293 cell samples i) not transfected, ii) MOCK plasmid transfected, and iii) triple-transfected with rAAV2-production plasmids were harvested 24 h and 72 h after transfection. In total 3384 proteins in all samples were identified revealing regulated proteins due to transfection and rAAV production.