RNAseq on induced endoreplicating S2 cells

RNA-Seq was used In order to evaluate the global transcriptomic changes of CycA dsRNA and Myb dsRNA induced endoreplicating cells (iECs) in DrosophilaS2-DGRC cells. CycA dsRNA and Myb dsRNA iECs mRNA levels were compared to control mitotic cycling cells treated in parallel with GFP dsRNA, in three biological replicates. The RNA-Seq results indicated that a switch from mitotic cycles to endoreplication is associated with up and down regulation of thousands of genes. Comparison of the CycA dsRNA and Myb dsRNA iEC transcriptomes revealed that they shared a total of 966 genes that were differentially expressed compared to mitotic cycling controls S2 cells treated with either GFP, CycA or Myb dsRNA were harvested 96 hours after dsRNA addition. After RNA isolation, poly-A tailed RNA was enriched and cDNA libraries were generated. RNAseq was done on three biological replicates using an Illumina NextSeq 500. CycA dsRNA and Myb dsRNA data sets were compared to GFP dsRNA control cells.