Next Generation Sequencing Facilitates Quantitative Analysis of Ocular Adnexal Lymphoma and inflammation Transcriptomes

Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to describe NGS-derived transcriptome profiling (RNA-seq) of ocular adnexal lymphomas (OALs). Gene expression profiling of RNA was performed on OAL and inflammation samples to identify pathway alterations and expression changes affected by clinical traits Overall design: OAL mRNA profiles of 20 samples, idiopathic orbital inflammation mRNA profiles of 4 samples, and reactive lymphoid hyperplaisa mRNA profiles of 6 samples from patients were generated by deep sequencing using Illumina Novaseq 6000 according to the manufacturer's direction. RNA was extracted from cryopreserved lymphoma tumor specimens. The sequence reads that passed quality filters were analyzed at the transcript isoform level.