The role of NRF2 in the effects of methionine restriction on the transcriptome of mouse livers

Dietary methionine restriction results in a robust activation of transcription factor, NRF2, and its target gene expression in the liver. In the current study, we observed liver-specific knockout NRF2 did not affect the physiological responses due to MR consumption. Therefore, there are other transcription factors compensate for the loss of NRF2 in the liver to mediate the effect of MR. The RNAseq experiment was comducted to compare the effect of MR diet in NRF2 WT and KO mice on hepatic transcriptome. Overall design: Comparison of the effect of methionine restriction (MR) on the hepatic transcriptome in NRF2 WT (each 6 samples from CON and MR fed mice) and NRF2 liver-specific KO mice (each 6 samples from CON and MR fed mice). The Control diets contain 0.86% methionine, the MR diets 0.17% methionine.