Transcription profiling of mouse small intestine at postnatal days 4,7,9,11,24 to investigate postnatal development of the small intestine

It was the purpose to analyze the changes in gene expression which occur in the mouse small intestine from the pre-weaning to the post-weaning stage. The gene expression was accordingly followed from postnatal day 4 to postnatal day 32. Experiment Overall Design: 6 time-points were defined corresponding to postnatal day 4, 7, 9, 11, 24 and 32. Ileal samples were taken from individual mice at each time-point. All layers of the small intestine were included in the sample. Three samples from individual mice were analyzed by hybridization to Affymetrix GeneChips.