soil metagenome Raw sequence reads

Soil microbial use efficiency of straw-C in the agricultural ecosystem, which is the proportion of straw-C microbes assimilate into new biosynthetic material relative to C lost out of the system as CO2, is critical in increasing soil organic C (SOC) content, and hence maintaining soil fertility and crop productivity. However, the effect of chemical structures of organic amendments (OAs) on microbial use efficiency of straw-C remains unclear. The effect of chemical structure of OAs on microbial use efficiency of straw-C was elucidated by a combination of 13C-straw labelling with high-throughput sequencing and pyrolysis-GC/MS. The bacterial and fungal community structure was elucidated by amplicon sequencing of the 16S rRNA and internal transcript spacer (ITS) genes, respectively.