Gut homeostasis alterations in Fisher 344 rats according to the iron form (haem vs. ferric citrate) and calcium source (mineral vs. dairy) in the diet: Supplementary Information

This dataset contains Supplementary Information related to a nutritional study whose main aims was to compare the impact of dietary calcium provided as calcium salts or dairy matrix on gut homeostasis perturbations by high haeminic or non-haeminic iron intakes. A 3-week intervention study was conducted using Fisher 344 rats. Composition of experimental diets, experimental design, sampling and analysis is given in the “Material and Methods” section of the main paper: “Olier et al. entitled “Calcium-rich dairy matrix protects better than mineral calcium against colonic luminal haem-induced alterations in male rats.” Detailed description of the dataset: The files deposited in this dataset are related to: 1. 16S rRNA gene amplicon sequence analysis described in the manuscript “MetaData_W2.csv” details about origin of the samples, the sequencing technology used and the associated file names. A link for raw sequences files for each sample. “W2_Calcium LDA3.tar” Output files of LDA analyses allowing to display faecal bacterial taxa with differential abundances (LDA>3 with a p-value of 0.05 or 0.01) according to diets. 2. Supplementary Tables of the manuscript Table S1: F-statistics and p values resulting from 2-way ANOVA analysis and Holm-Šídák's multiple comparison tests. Table S2: Mucosal gene expression related to inflammation and detoxification pathways. Table S3: List of genes and primers sequences used in this study. Table S4: Bacterial taxonomy of species found in fecal microbiota of rats. 3. Supplementary Figures of the manuscript The file entitled “Supplementary Figures.pdf” includes: Figure S1: Impact of dietary calcium content (Control vs. Mineral vs. Dairy Calcium) on abundance of faecal bacterial taxa in rats fed ferric citrate or haemoglobin. Figure S2: Impact of dietary iron form (Ferric citrate vs. Haemoglobin) on abundance of faecal bacterial taxa in rats fed variable calcium intake. Figure S3: Haemoglobin-induced changes in faecal microbiota not normalized by the addition of dietary calcium, regardless of its origin. Figure S4: Bacterial communities not affected by dietary haemoglobin but differentially influenced by dietary calcium.