nsp14 binds nsp12

This COVID-19 event has been created by a combination of computational inference (see https://reactome.org/documentation/inferred-events) from SARS-CoV-1 data and manual curation, as described in the summation for the overall SARS-CoV-2 infection pathway.<br><br>Non-structural protein 14 (nsp14) of the human SARS coronavirus is a bifunctional enzyme bearing 3'-5 exoribonuclease activity involved in replication fidelity and RNA cap N7-guanine methyltransferase activity involved in 5'-RNA capping. nsp14 binds to the minimal replication and transcription complex (RTC), composed of nsp7, nsp8, and nsp12, by directly binding to nsp12 (the main RNA-dependent RNA polymerase). Binding of nsp14 does not affect the processivity of the RTC (Minskaia et al. 2006, Subissi et al. 2014).

本COVID-19事件系由SARS-CoV-1数据经计算推理（参见https://reactome.org/documentation/inferred-events）及人工编纂相结合而成，具体编纂过程详述于SARS-CoV-2感染途径概述中。人SARS冠状病毒的非结构蛋白14（nsp14）是一种双功能酶，具备3'-5'外切核糖核酸酶活性，参与复制忠实度，并具有5'-RNA帽N7鸟嘌呤甲基转移酶活性，参与5'-RNA帽的形成。nsp14通过直接结合nsp12（主要的RNA依赖性RNA聚合酶）与最小的复制和转录复合体（RTC），RTC由nsp7、nsp8和nsp12组成。nsp14的结合不会影响RTC的进程性（Minskaia等，2006年，Subissi等，2014年）。