T47D RNA-seq and ChrRNA-seq data. T47D RNA-seq and ChrRNA-seq data

We performed genome-wide RNA-seq and ChrRNA-seq experiments in T47D cell lines after PADI2 knock down . Both the mRNA and chromatin RNA seq peformed in sicontrol and siPADI2 as in biological replicates . Also we performed mRNA seq in T47D cells expressing HA Tagged amanitin resistant wild type in comparison to R1810A mutant form of RNAP2. Overall design: We designed experiment to perform mRNA sequencing in T47D after PADI2 knock down as in biological replicate experiemnt . Given the fact we found significant number of genes differentailly expressed after PADI2 knockdown in T47D cells, we designed to perform Chromatin RNA seq in T47D cells after PADI2 knock down as in biological replicates. We find that PADI2 deiminates R1810 at CTD (C-terminal domian) of RNAP2 , So in order to analyse the mRNA gene expression level in T47D cells expressing amanitin resistant HA - tagged wild type and mutant form of RNAP2 , we performed mRNA seq as in biological replicate in T47D cells expressing amanitin resistant HA - tagged wild type and mutant form of RNAP2.