Expression Profile Analysis of Long non-coding RNAs in Mouse Liver

In the present study, the lncRNA expression profile of mouse fetal and adult liver was analyzed by ArrayStar LncRNA Array 3.0. Differentially expressed LncRNAs with statistical significance were identified through Volcano Plot filtering between two groups. Pathway analysis and GO analysis were applied to determine the roles of these differentially expressed lncRNAs played in these biological pathways or GO terms. Finally, Hierarchical Clustering was performed to show the distinguishable LncRNAs expression pattern among samples. The total RNA from C57/BL6 mouse fetal (embryo-E18) and adult (2-months old) liver were extracted and RNA integrity was assessed by standard denaturing agarose gel electrophoresis and Bioanalyzer. For lncRNA array analysis, ArrayStar Mouse LncRNA Microarray V3.0 was employed. Three independent experiments were performed at each condition (liver-E18 vs. liver-2months). To identify differentially expressed LncRNAs with statistical significance, we performed a Volcano Plot filtering between the two groups (Fold Change >= 2.0, P-value <= 0.05).