KDM5C-mediated BRD4 recruitment is essential for active states of enhancers and BET inhibitor sensitivity [RNA-seq]

To investigate the effect of the KDM5C depletion on transcription, we established HeLa cell lines in which KDM5C has been knocked off. To explore the effect of synergistic lethal action of KDM5C PROTAC drug and BETi on transcription, we treated HeLa cells with JQ1, or compound 3b, or in combination. Comparative gene expression profiling analysis of RNA-seq data for HeLa parental and sgKDM5C cell lines, as well as parental and three different treatment cells (JQ1, compound 3b, and in combination).