S1 Table.xlsx

A hand-held MyotonPRO device (MyotonPRO, Myoton Ltd, Estonia) was programmed to perform a series of scans at regular intervals every second, each of which would consist of a short-duration (15 ms) impulse using a minimum amount of mechanical force (0.4 N) and gentle precompression (0.18 N). Based on the mechanical dynamic response method, it will provide a subsequent computation of parameters characterizing the PT's biomechanical (tissue/muscle tone, dynamic stiffness, and decrement) and viscoelastic properties (mechanical stress relaxation time and ratio of relaxation and deformation time). The multi-scan measurement consists of five individual measurements (the instrument generated five rapid mechanical impulses) and is automatically reported as the average of these observations. Multi-scan measurements were conducted five times for the three different stifle angles on both legs and groups. Following this, analysis was performed using the average of the five repetitions. Moreover, to prevent misinterpretation due to the stability factor, the leg that rested on the examination table was utilized as the standard for measuring. The same dog was subjected to two experimental procedures. All dogs were evaluated prior to rocuronium administration; this group is the control group. After completing all evaluations, rocuronium was delivered to the same dog, after which they represented the muscle relaxation group and underwent evaluations similar to those previously mentioned.The sample under consideration comprises five distinct dogs, denoted as A1, A2, A3, A4, and A5. Each of these canine subjects underwent a rigorous assessment protocol involving a total of five repetitions, with data collection conducted during each repetition. It is important to note that the data collected pertains to two distinct temporal states: "PRE," signifying measurements taken before being given by muscle relaxant, and "PO," representing measurements taken after the muscle relaxant administration. Furthermore, within each measurement session, the dogs were positioned in one of three specific postures: "N," denoting the normal or standing position; "E," indicating an extended position; and "F," representing a flexed position. These postures were carefully controlled to ensure consistency across all measurements, thereby enhancing the reliability and validity of the data acquired during this rigorous assessment process.