Analysis of some typical mutations in genes inducing suppression of frameshift mutations.

a)Monitored as the ability to suppress the his-allele the mutant was selected to suppress. Growth of mutants on a plate lacking His following and incubation at 37°C for 4–6 days. The parental strain, which has no suppressor mutation but the indicated his-allele, did not grow on plates without His.b)The suppression was monitored as the ability to suppress the CCC-CAA-UAG sequence placed in front of the lacZ gene (See M–M).c)Plasmids (See figure 4) used to determine the amino acid sequence at the frameshifting site and the last amino acid in the zero frame is indicated in parenthesis.d)P-site according to Qian et al 1998 [34].e)P-site according to [35].f)According to [36].g)According to [69].h)According to [16].i)P-site since these mutants also lack the s2-group of mnm5s2U similar to the mnmA mutants.j)P-site since mnmG mutants like mnmE mutants lack the mnm5-side chain of mnm5s2U.k)According to [71].pUST136: contains the metT operon in which glnU is present. It also contains the gene miaB. See Esberg et al 1999 [85].pCBS4 (glnS+) contains the glnS+ wild type allele.