Human T-cells EVs LC-MSMS - Extracellular Vesicle Subtypes Released From Activated or Apoptotic T-Lymphocytes Carry a Specific and Stimulus-Dependent Protein Cargo

As it is likely that different stimuli promote the release of distinct EV populations, we analyzed EVs from human lymphocytes considering the respective release stimuli (activation vs. apoptosis induction). Morphology and size were analyzed by electron microscopy and nanoparticle tracking analysis. The protein content of these vesicles was analyzed by bidimensional gel electrophoresis followed by mass spec and western blot.