One step assembly of promoter, GOI and terminator by Hot Fusion reaction.
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A binary vector was digested with SfaAI to release the lacZ gene from the cloning site, heat-inactivated and used directly in this study. Blue colonies indicate the parental vector (background) and white colonies indicate potential positive clones. The last column indicates true positive clones based on PCR screening of 8 clones per construct. One step assembly of promoter, GOI and terminator by Hot Fusion reaction.
创建时间：
2014-12-31
