Protease activity; one unit of activity is defined as the amount of enzyme required to cause an increase in O.D. by 0.01 at 660 nm/min (a).

Indirect hemolytic activity; it is expressed as a percent of hemolysis (b). Direct hemolytic activity; it is expressed as a percent of hemolysis (c). L-amino acid oxidase activity; one unit of activity is defined as the amount of enzyme required to cause an increase in O.D. by 0.001 at 440 nm/min (d). 5’-nucleotidase activity; one unit of activity is expressed in terms of the release of inorganic phosphorus in μ moles/min/μg of protein (e). The data is presented as Mean ± SEM (n = 3).